Regulatory
PclpB

Part:BBa_K3773006:Design

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-14)


clpB promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was successfully used as in a similar circuit used to detect heat shock response through GFP fluorescence.


Source

Kitagawa, M. A. S. A. N. A. R. I., Wada, C., Yoshioka, S., & Yura, T. (1991). Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32). Journal of Bacteriology, 173(14), 4247-4253.

Squires, C. L., Pedersen, S., Ross, B. M., & Squires, C. (1991). ClpB is the Escherichia coli heat shock protein F84. 1. Journal of bacteriology, 173(14), 4254-4262.

Cha, H. J., Srivastava, R., Vakharia, V. N., Rao, G., & Bentley, W. E. (1999). Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells. Applied and Environmental Microbiology, 65(2), 409-414.


References